MA5-23835

antibody from Invitrogen Antibodies

Targeting: CCL22 A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23835

Provider

Invitrogen Antibodies

Product name

MDC Monoclonal Antibody (57226)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In ELISAs, this antibody does notcross-react with recombinant mouse (rm) CCL17, rhCCL17, or rhCCL21. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

57226

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of MDC is shown by the decrease in fluorescence (Chemotaxis measured by Resazurin) with increasing concentrations of MDC Antibody (Product # MA5-23754).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of MDC in BaF3 mouse pro‚B cell line transfected with human CCR4. Samples were incubated in MDC monoclonal antibody (Product # MA5-23835). Recombinant Human CCL22/MDC chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL22/MDC (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL22/MDC Monoclonal Antibody. The ND50 is typically 0.6-3.0 µg/mL.