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Learn500-P107-1MG
antibody from Invitrogen Antibodies
Targeting: CCL22
A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P107-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDC Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GPYGANMEDS VCCRDYVRYR LPLRVVKHFY WTSDSCPRPG VVLLTFRDKE ICADPRVPWV KMILNKLSQ. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MDC (CCL22). Anti-Human MDC (CCL22)-specific antibody was purified by affinity chromatography employing an immobilized Human MDC (CCL22) matrix. Sandwich ELISA: To detect Human MDC (CCL22) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MDC (CCL22) (500-P107Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MDC (CCL22). Western Blot: To detect Human MDC (CCL22) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Regulatory T Cell Apoptosis during Preeclampsia May Be Prevented by Gal-2.
Cross talk between follicular Th cells and tumor cells in human follicular lymphoma promotes immune evasion in the tumor microenvironment.
Soluble factors from T cells inhibiting X4 strains of HIV are a mixture of β chemokines and RNases.
Quantification of the chemokines CCL17 and CCL22 in human colorectal adenocarcinomas.
Diverse and potent chemokine production by lung CD11bhigh dendritic cells in homeostasis and in allergic lung inflammation.
Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.
Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
Expression of CCL28 by Reed-Sternberg cells defines a major subtype of classical Hodgkin's disease with frequent infiltration of eosinophils and/or plasma cells.
Macrophage-derived chemokine (MDC/CCL22) and CCR4 are involved in the formation of T lymphocyte-dendritic cell clusters in human inflamed skin and secondary lymphoid tissue.
Meister S, Hahn L, Beyer S, Mannewitz M, Perleberg C, Schnell K, Anz D, Corradini S, Schmoeckel E, Mayr D, Hasbargen U, Zati Zehni A, Mahner S, Jeschke U, Kolben T
International journal of molecular sciences 2022 Feb 7;23(3)
International journal of molecular sciences 2022 Feb 7;23(3)
Cross talk between follicular Th cells and tumor cells in human follicular lymphoma promotes immune evasion in the tumor microenvironment.
Rawal S, Chu F, Zhang M, Park HJ, Nattamai D, Kannan S, Sharma R, Delgado D, Chou T, Lin HY, Baladandayuthapani V, Luong A, Vega F, Fowler N, Dong C, Davis RE, Neelapu SS
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6681-93
Journal of immunology (Baltimore, Md. : 1950) 2013 Jun 15;190(12):6681-93
Soluble factors from T cells inhibiting X4 strains of HIV are a mixture of β chemokines and RNases.
Cocchi F, DeVico AL, Lu W, Popovic M, Latinovic O, Sajadi MM, Redfield RR, Lafferty MK, Galli M, Garzino-Demo A, Gallo RC
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 3;109(14):5411-6
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 3;109(14):5411-6
Quantification of the chemokines CCL17 and CCL22 in human colorectal adenocarcinomas.
Wågsäter D, Dienus O, Löfgren S, Hugander A, Dimberg J
Molecular medicine reports 2008 Mar-Apr;1(2):211-7
Molecular medicine reports 2008 Mar-Apr;1(2):211-7
Diverse and potent chemokine production by lung CD11bhigh dendritic cells in homeostasis and in allergic lung inflammation.
Beaty SR, Rose CE Jr, Sung SS
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1882-95
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1882-95
Nuclear and cytoplasmic AID in extrafollicular and germinal center B cells.
Cattoretti G, Büttner M, Shaknovich R, Kremmer E, Alobeid B, Niedobitek G
Blood 2006 May 15;107(10):3967-75
Blood 2006 May 15;107(10):3967-75
Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
Mateo T, Abu Nabah YN, Abu Taha M, Mata M, Cerdá-Nicolás M, Proudfoot AE, Stahl RA, Issekutz AC, Cortijo J, Morcillo EJ, Jose PJ, Sanz MJ
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5577-86
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5577-86
Expression of CCL28 by Reed-Sternberg cells defines a major subtype of classical Hodgkin's disease with frequent infiltration of eosinophils and/or plasma cells.
Hanamoto H, Nakayama T, Miyazato H, Takegawa S, Hieshima K, Tatsumi Y, Kanamaru A, Yoshie O
The American journal of pathology 2004 Mar;164(3):997-1006
The American journal of pathology 2004 Mar;164(3):997-1006
Macrophage-derived chemokine (MDC/CCL22) and CCR4 are involved in the formation of T lymphocyte-dendritic cell clusters in human inflamed skin and secondary lymphoid tissue.
Katou F, Ohtani H, Nakayama T, Ono K, Matsushima K, Saaristo A, Nagura H, Yoshie O, Motegi K
The American journal of pathology 2001 Apr;158(4):1263-70
The American journal of pathology 2001 Apr;158(4):1263-70
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MDC (CCL22) by Western Blot analysis MDC Polyclonal Antibody (Product # 500-P107-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human MDC (CCL22) by Western Blot analysis MDC Polyclonal Antibody (Product # 500-P107-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MDC (CCL22) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human MDC (CCL22) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of MDC Polyclonal Antibody (Product # 500-P107-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech MDC Polyclonal Antibody, Biotin (Product # 500-P107BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MDC (CCL22).
