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antibody from R&D Systems
Targeting: CCL22
A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF336 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL22/MDC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified from egg yolks. Detects human CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse MDC and recombinant human MPIF-1 is observed.
- Reactivity
- Human
- Host
- Chicken/Avian
- Conjugate
- Unconjugated
- Antigen sequence
O00626- Isotype
- IgY
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression and regulation of chemokines in murine and human type 1 diabetes.
Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D
Diabetes 2012 Feb;61(2):436-46
Diabetes 2012 Feb;61(2):436-46
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody. Recombinant Human CCL22/MDC (Catalog # 336-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL22/MDC (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-Human CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF336). The ND50 is typically 0.4-2.0 µg/mL.
