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LearnIC3361P
antibody from R&D Systems
Targeting: CCL22
A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- IC3361P - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL22/MDC PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant cotton rat CCL3, 4, 5, recombinant human CCL1, 2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma , 11, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 8, 9/10/MIP-1 gamma , 11, 12, 17, 19, 21, 22, 24, 25, 27, 28, or recombinant rat CCL20.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
O00626.1- Isotype
- IgG
- Antibody clone number
- 57203
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Identification of human NK17/NK1 cells.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.
Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic SS, Caragol I, Baumann U, Klein C, Welte K, Hansen G
The Journal of allergy and clinical immunology 2014 Jan;133(1):172-80.e1-10
The Journal of allergy and clinical immunology 2014 Jan;133(1):172-80.e1-10
Identification of human NK17/NK1 cells.
Pandya AD, Al-Jaderi Z, Høglund RA, Holmøy T, Harbo HF, Norgauer J, Maghazachi AA
PloS one 2011;6(10):e26780
PloS one 2011;6(10):e26780
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.
Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H
International journal of cancer 2008 May 15;122(10):2286-93
International journal of cancer 2008 May 15;122(10):2286-93
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCL22/MDC in Human Dendritic Cells by Flow Cytometry. Human dendritic cells treated with LPS, Recombinant Human TNF-alpha (Catalog # 210-TA), and Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) were stained with Mouse Anti-Human CCL22/MDC PE-conjugated Monoclonal Antibody (Catalog # IC3361P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
