PA1-29224

antibody from Invitrogen Antibodies

Targeting: CX3CL1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, SCYD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-29224

Provider

Invitrogen Antibodies

Product name

CX3CL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot detection of hFractalkine in transfected (1) and untransfected (2) cos-7 cells using CX3CL1 Polyclonal Antibody (Product # PA1-29224).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CX3CL1 Polyclonal Antibody (Product # PA1-29224) and a 85 kDa band corresponding to soluble cleaved CX3CL1 was observed across tested cell line. Conditioned media (20 µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF-alpha, 10 ng/ml for 12 hrs (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry anlaysis using HepG2 cells stained withCX3CL1 Polyclonal Antibody (Product # PA1-29224), green; counterstained for tubulin, red and nuclei, blue.