H00010875-M01

antibody from Novus Biologicals

Targeting: FGL2 pT49, T49

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Functional assay (Data presented on provider website)

Antibody Data

Product number

H00010875-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00010875-M01, RRID:AB_669190

Product name

Mouse Monoclonal FGL2/Fibroleukin Antibody

Antibody type

Monoclonal

Description

Protein A purified. FGL2 (6D9)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Von Willebrand factor protects against acute CCl₄-induced hepatotoxicity through phospho-p38 MAPK signaling pathway inhibition.

Sun HJ, Chen J, Zhang H, Ni B, van Velkinburgh JC, Liu Y, Wu YZ, Yang X
Immunologic research 2017 Oct;65(5):1046-1058

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Adenovirus-mediated artificial microRNA against human fibrinogen like protein 2 inhibits hepatocellular carcinoma growth.

Wang M, Liu J, Xi D, Luo X, Ning Q
The journal of gene medicine 2016 Jul;18(7):102-11

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Sodium tanshinone IIA sulfonate ameliorates experimental coronary no-reflow phenomenon through down-regulation of FGL2.

Long R, You Y, Li W, Jin N, Huang S, Li T, Liu K, Wang Z
Life sciences 2015 Dec 1;142:8-18

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Novel antibody against a glutamic acid-rich human fibrinogen-like protein 2-derived peptide near Ser91 inhibits hfgl2 prothrombinase activity.

Li WZ, Wang J, Long R, Su GH, Bukhory DK, Dai J, Jin N, Huang SY, Jia P, Li T, Fan C, Liu K, Wang Z
PloS one 2014;9(4):e94551

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Soluble FGL2, a novel effector molecule of activated hepatic stellate cells, regulates T-cell function in cirrhotic patients with hepatocellular carcinoma.

Sun Y, Xi D, Ding W, Wang F, Zhou H, Ning Q
Hepatology international 2014 Oct;8(4):567-75

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C5a/C5aR pathway is essential for the pathogenesis of murine viral fulminant hepatitis by way of potentiating Fgl2/fibroleukin expression.

Xu GL, Chen J, Yang F, Li GQ, Zheng LX, Wu YZ
Hepatology (Baltimore, Md.) 2014 Jul;60(1):114-24

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Correlation of fibrinogen-like protein 2 with disease progression in patients with severe acute pancreatitis.

Ye X, Huai J, Chen R, Ding J, Chen Y, Cai Z
Experimental and therapeutic medicine 2014 Jan;7(1):85-89

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Intestinal and peripheral fibrinogen-like protein 2 expression in inflammatory bowel disease.

Dong X, Ye X, Chen X, Chen T, Xie S, Li Q, Lin X, Huang Z
Digestive diseases and sciences 2014 Apr;59(4):769-77

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Combined adenovirus-mediated artificial microRNAs targeting mfgl2, mFas, and mTNFR1 protect against fulminant hepatic failure in mice.

Xi D, Wang M, Ye H, Luo X, Ning Q
PloS one 2013;8(11):e82330

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FGL2/fibroleukin mediates hepatic reperfusion injury by induction of sinusoidal endothelial cell and hepatocyte apoptosis in mice.

Selzner N, Liu H, Boehnert MU, Adeyi OA, Shalev I, Bartczak AM, Xue-Zhong M, Manuel J, Rotstein OD, McGilvray ID, Grant DR, Phillips MJ, Levy GA, Selzner M
Journal of hepatology 2012 Jan;56(1):153-9

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The FGL2/fibroleukin prothrombinase is involved in alveolar macrophage activation in COPD through the MAPK pathway.

Liu Y, Xu S, Xiao F, Xiong Y, Wang X, Gao S, Yan W, Ning Q
Biochemical and biophysical research communications 2010 May 28;396(2):555-61

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Expression of prothrombin and protease activated receptors in human myometrium during pregnancy and labor.

O'Brien M, Morrison JJ, Smith TJ
Biology of reproduction 2008 Jan;78(1):20-6

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Targeted deletion of fgl2 leads to impaired regulatory T cell activity and development of autoimmune glomerulonephritis.

Shalev I, Liu H, Koscik C, Bartczak A, Javadi M, Wong KM, Maknojia A, He W, Liu MF, Diao J, Winter E, Manuel J, McCarthy D, Cattral M, Gommerman J, Clark DA, Phillips MJ, Gorczynski RR, Zhang L, Downey G, Grant D, Cybulsky MI, Levy G
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 1;180(1):249-60

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Targeted deletion of fgl2 leads to impaired regulatory T cell activity and development of autoimmune glomerulonephritis.

Shalev I, Liu H, Koscik C, Bartczak A, Javadi M, Wong KM, Maknojia A, He W, Liu MF, Diao J, Winter E, Manuel J, McCarthy D, Cattral M, Gommerman J, Clark DA, Phillips MJ, Gorczynski RR, Zhang L, Downey G, Grant D, Cybulsky MI, Levy G
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 1;180(1):249-60

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Analysis of FGL2 expression in transfected 293T cell line by FGL2 monoclonal antibody (M01), clone 6D9.Lane 1: FGL2 transfected lysate(50 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - FGL2 monoclonal antibody (M01), clone 6D9 Analysis of FGL2 expression in HeLa.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Detection limit for recombinant GST tagged FGL2 is approximately 0.1ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: FGL2/Fibroleukin Antibody (6D9) [H00010875-M01] - Analysis of monoclonal antibody to FGL2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 0.5 ug/ml.