11-0982-42
antibody from Invitrogen Antibodies
Targeting: SLC3A2
4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 11-0982-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD98hc Monoclonal Antibody (5E5), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 5E5 recognizes human CD98hc protein also called CD98 heavy chain, FRP-1 (fusion regulatory protein-1 ) or 4F2. CD98 is a disulfide-linked complex composed of a heavy chain (a Type II membrane protein) and a light chain of which there are more than six different chains (LAT1, LAT2, y+LAT1, y+LAT2, Asc-1, xCT). CD98 can associate directly or indirectly with beta 1 integrins, EpCAM and CD147 (EMMPRIN). The function of CD98 is thought to involve amino acid transport, cell fusion concomitantly with increased plasma membrane expression and integrin mediated adhesion. Expression of CD98 is broad including non-hematopoietic cells. In peripheral blood cells, monocytes expression is high while lymphocytes have intermediate levels and granulocytes express very little. Applications Reported: This 5E5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 5E5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 5E5
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references CD138 and CD31 Double-Positive Cells Comprise the Functional Antibody-Secreting Plasma Cell Compartment in Primate Bone Marrow.
The tryptophan metabolite picolinic acid suppresses proliferation and metabolic activity of CD4+ T cells and inhibits c-Myc activation.
Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes.
Martinez-Murillo P, Pramanik L, Sundling C, Hultenby K, Wretenberg P, SpÄngberg M, Karlsson Hedestam GB
Frontiers in immunology 2016;7:242
Frontiers in immunology 2016;7:242
The tryptophan metabolite picolinic acid suppresses proliferation and metabolic activity of CD4+ T cells and inhibits c-Myc activation.
Prodinger J, Loacker LJ, Schmidt RL, Ratzinger F, Greiner G, Witzeneder N, Hoermann G, Jutz S, Pickl WF, Steinberger P, Marculescu R, Schmetterer KG
Journal of leukocyte biology 2016 Apr;99(4):583-94
Journal of leukocyte biology 2016 Apr;99(4):583-94
Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes.
Haynes BF, Hemler ME, Mann DL, Eisenbarth GS, Shelhamer J, Mostowski HS, Thomas CA, Strominger JL, Fauci AS
Journal of immunology (Baltimore, Md. : 1950) 1981 Apr;126(4):1409-14
Journal of immunology (Baltimore, Md. : 1950) 1981 Apr;126(4):1409-14
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD98hc FITC (filled histogram). Cells in the monocyte gate were used for analysis.
- Conjugate
- Green dye