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antibody from Invitrogen Antibodies
Targeting: SLC3A2
4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE
Western blot
ELISA
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- PA5-118266 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD98 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD98 Polyclonal Antibody (Product # PA5-118266) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: Raji Whole Cell Lysate, Lane D: Molt-4 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the technique. Performed under reducing conditions. Predicted band size: 68 kDa. Observed band size: 75 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of CD98 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD98 Polyclonal Antibody (Product # PA5-118266, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and cell membrane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of CD98 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with CD98 Polyclonal Antibody (Product # PA5-118266, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and cell membrane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of CD98 in Lane A: 0.5 mg HEPG2 Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-118266) with a dilution of 1:100 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 72 kDa , Observed band size : 72 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD98 Immunoprecipitation using: Lane A: 0.5 mg HEPG2 Whole Cell Lysate 4 µL with CD98 Polyclonal Antibody (Product # PA5-118266) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CD98 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa. Observed band size: 72 kDa.
