PA5-21547

antibody from Invitrogen Antibodies

Targeting: SLC3A2 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21547

Provider

Invitrogen Antibodies

Product name

CD98 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Dog (83%), Rabbit (83%), Bovine (81%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.19 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD98/SLC3A2 using 30 µg of MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD98/SLC3A2 polyclonal antibody (Product # PA5-21547) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD98 was performed by separating 30 µg of A431 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD98 Polyclonal Antibody (Product # PA5-21547) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with PMA (10 ng/mL for 15hr) (Lane 2), Jurkat treated with both PMA (10 ng/mL) and Ionomycin (1 µg/mL) together for 15hr (Lane 3), HeLa (Lane 4) and SK-OV-3 (Lane 5). The blot was probed with Anti-CD98 Polyclonal Antibody (Product # PA5-21547, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 68 kDa band corresponding to CD98 was detected across all the cell lines and also the band was enhanced in Jurkat cell line upon PMA and PMA treated with Ionomycin. Two uncharacterized bands were observed in HeLa and SK-OV-3.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD98 was performed using log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD98 Polyclonal Antibody (Product # PA5-21547) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing Membranous localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded SCC15 xenograft, using CD98 (Product # PA5-21547) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.