PA5-23661
antibody from Invitrogen Antibodies
Targeting: SLC3A2
4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-23661 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD98 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a SLC3A2 polyclonal antibody (Product # PA5-23661) in A2058 (lane 1), A375 (lane 2) cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with PMA (10 ng/mL for 15hr) (Lane 2), Jurkat treated with both PMA (10 ng/mL) and Ionomycin (1 µg/mL) together for 15hr (Lane 3), HeLa (Lane 4) and SK-OV-3 (Lane 5). The blot was probed with Anti-CD98 Polyclonal Antibody (Product # PA5-23661, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 68 kDa band corresponding to CD98 was detected across all the cell lines and also the band was enhanced in Jurkat cell line upon PMA and PMA treated with Ionomycin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human placenta tissue using a SLC3A2 polyclonal antibody (Product # PA5-23661), followed by HRP-conjugated secondary antibody and DAB staining.