PA5-23661

antibody from Invitrogen Antibodies

Targeting: SLC3A2 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23661

Provider

Invitrogen Antibodies

Product name

CD98 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a SLC3A2 polyclonal antibody (Product # PA5-23661) in A2058 (lane 1), A375 (lane 2) cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with PMA (10 ng/mL for 15hr) (Lane 2), Jurkat treated with both PMA (10 ng/mL) and Ionomycin (1 µg/mL) together for 15hr (Lane 3), HeLa (Lane 4) and SK-OV-3 (Lane 5). The blot was probed with Anti-CD98 Polyclonal Antibody (Product # PA5-23661, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 68 kDa band corresponding to CD98 was detected across all the cell lines and also the band was enhanced in Jurkat cell line upon PMA and PMA treated with Ionomycin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human placenta tissue using a SLC3A2 polyclonal antibody (Product # PA5-23661), followed by HRP-conjugated secondary antibody and DAB staining.