PA5-57720

antibody from Invitrogen Antibodies

Targeting: MATR3 KIAA0723, MGC9105, MPD2, VCPDM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-57720

Provider

Invitrogen Antibodies

Product name

MATR3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LKRRRTEEGP TLSYGRDGRS ATREPPYRVP RDDWEEKRHF RRDSFDDRGP SLNPVLDYDH GSRSQESGYY DRMDYEDDRL RDGERCRDD Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.08 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MATR3 in human cell line SH-SY5Y using a MATR3 Polyclonal Antibody (Product # PA5-57720).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Matrin-3 was performed by loading 20 µg of WT (lane 1) and MATR3 CRISPR KO (lane 2) HAP1 cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. MATR3 was detected above 100 kDa (designated by the black arrow) using a MATR3 polyclonal antibody (Product # PA5-57720) at a dilution of 1:200 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 65-6120) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MATR3 in mouse cell line NIH-3T3 and rat cell line NBT-II using a MATR3 Polyclonal Antibody (Product # PA5-57720).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of MATR3 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a MATR3 Polyclonal Antibody (Product # PA5-57720).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of MATR3 was performed using HAP1 wild-type and MATR3 KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the MATR3 polyclonal antibody (Product # PA5-57720) at a 1:2,000 dilution overnight at 4°C. Secondary antibody incubation was performed using 1 µg/mL of Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 antibody (Product # A21429) for 1 hr. Imaging was performed with a 40X oil objective and analysis was performed using Image J. Cell image represents a single focal plane; WT and KO cells are outlined with a yellow (WT) or magenta (KO) dashed line. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MATR3 in human cerebral cortex using MATR3 Polyclonal Antibody (Product # PA5-57720) shows strong nuclear positivity in neurons.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MATR3 in human endometrium using MATR3 Polyclonal Antibody (Product # PA5-57720) shows moderate nuclear positivity in glandular cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MATR3 in human lymph node using MATR3 Polyclonal Antibody (Product # PA5-57720) shows moderate to strong nuclear positivity in lymphoid cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MATR3 in human pancreas using MATR3 Polyclonal Antibody (Product # PA5-57720) shows moderate nuclear positivity in exocrine glandular cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MATR3 was performed on HAP1 cell lysates. Antibody-bead conjugates were prepared by adding 2 µg of MATR3 polyclonal antibody (Product # PA5-57720) with 30 µL of protein A-Sepharose beads and rocked overnight at 4°C. 1 mg of lysate was incubated with an antibody-bead conjugate for 2 hours at 4°C. Following centrifugation and multiple washes, 10% starting material (SM), 10% unbound fraction (UB) and immunoprecipitated fraction (IP) were processed for immunoblot using the same antibody. Ponceau stained transfer of blot is shown. Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.