A300-591A

antibody from Invitrogen Antibodies

Targeting: MATR3 KIAA0723, MGC9105, MPD2, VCPDM

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-591A

Provider

Invitrogen Antibodies

Product name

Matrin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Matrin 3 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T, 50 µg) and mouse NIH 3T3 (M, 50 µg) cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody (Product # A300-591A; lot A300-591A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Matrin 3 by western blot (H & M) and immunoprecipitation (H). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 1/4 of IP loaded), 293T (T, 50 µg for WB) and mouse NIH3T3 (M, 50 µg for WB) cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody BL2526 (Cat. No. A300-591A) used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used in IP at 3 µg/mg lysate (B). Matrin 3 was also immunoprecipitated using rabbit anti-Matrin 3 antibodies BL2524 and BL2525 (Cat. No. A300-590A) at 3 µg/mg lysate. For detecting immunoprecipitated Matrin 3, ReliaBLOT™ reagents and procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Matrin 3 by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- Matrin 3 (Cat. No. A300-591A Lot1) used at a dilution of 1:1,000 (0.2µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human expndtw-6 Matrin 3 by immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Matrin 3 (Cat. No. A300-591A Lot1) used at a dilution of 1:400 (0.5µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Matrin 3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 1/4 of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Matrin 3 antibody (Product # A300-591A; lot A300-591A-1) used for IP at 3 µg/mg lysate. Matrin 3 was also immunoprecipitated using rabbit anti-Matrin 3 antibodies (BL2524 and Product # A300-591A). For blotting immunoprecipitated Matrin 3 (Product # A300-591A) was used at 1 µg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.