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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP1312a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP1312a, RRID:AB_2143989
- Product name
- MCL1 Antibody (BH3 Domain Specific)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references DNA methylation-regulated miR-193a-3p dictates resistance of hepatocellular carcinoma to 5-fluorouracil via repression of SRSF2 expression.
P-glycoprotein enhances radiation-induced apoptotic cell death through the regulation of miR-16 and Bcl-2 expressions in hepatocellular carcinoma cells.
Characterization of the TCL-1 transgenic mouse as a preclinical drug development tool for human chronic lymphocytic leukemia.
Enforced Bcl-2 expression overrides serum and feeder cell requirements for mouse embryonic stem cell self-renewal.
Ma K, He Y, Zhang H, Fei Q, Niu D, Wang D, Ding X, Xu H, Chen X, Zhu J
The Journal of biological chemistry 2012 Feb 17;287(8):5639-49
The Journal of biological chemistry 2012 Feb 17;287(8):5639-49
P-glycoprotein enhances radiation-induced apoptotic cell death through the regulation of miR-16 and Bcl-2 expressions in hepatocellular carcinoma cells.
Tsang TY, Tang WY, Chan JY, Co NN, Au Yeung CL, Yau PL, Kong SK, Fung KP, Kwok TT
Apoptosis : an international journal on programmed cell death 2011 May;16(5):524-35
Apoptosis : an international journal on programmed cell death 2011 May;16(5):524-35
Characterization of the TCL-1 transgenic mouse as a preclinical drug development tool for human chronic lymphocytic leukemia.
Johnson AJ, Lucas DM, Muthusamy N, Smith LL, Edwards RB, De Lay MD, Croce CM, Grever MR, Byrd JC
Blood 2006 Aug 15;108(4):1334-8
Blood 2006 Aug 15;108(4):1334-8
Enforced Bcl-2 expression overrides serum and feeder cell requirements for mouse embryonic stem cell self-renewal.
Yamane T, Dylla SJ, Muijtjens M, Weissman IL
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 1;102(9):3312-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 1;102(9):3312-7
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Mcl-1 BH3 Domain Pab (Cat. #AP1312a) in Ramos cell line lysates (35ug/lane). Mcl-1-BH3(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ZR-75-1 cells using MCL1 Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
