Explore
Validate
Learn
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-9047-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCL-1 Monoclonal Antibody (LVUBKM), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This LVUBKM monoclonal antibody recognizes human and mouse myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 is an anti-apoptotic member of the Bcl-2 family of proteins important for regulation of cell survival/apoptosis. Mcl-1 is primarily localized to the outer membrane of mitochondria where it prevents cytochrome c release via dimerization with other Bcl-2 family members such as Bim. Although it is expressed in both immune and non-immune cells, highest levels of Mcl-1 expression are seen in hematopoietic lineage cells. PI3K activation of AKT results in destabilization and degradation of GSK3 beta, which prevents phosphorylation of Mcl-1 on S159 and its subsequent ubiquitnation and degradation. Mice conditionally lacking Mcl-1 in lymphocytes showed that Mcl-1 is essential during early lymphoid development and for the maintenance of mature lymphocytes. Applications Reported: This LVUBKM antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections and intracellular staining followed by flow cytometric analysis. (Fluorochrome-conjugated LVUBKM is recommended for use in intracellular flow cytometry.). Applications Tested: This LVUBKM antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LVUBKM
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Enhanced stability of Mcl1, a prosurvival Bcl2 relative, blunts stress-induced apoptosis, causes male sterility, and promotes tumorigenesis.
IL-15 regulates Bcl-2 family members Bim and Mcl-1 through JAK/STAT and PI3K/AKT pathways in T cells.
Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1.
Okamoto T, Coultas L, Metcalf D, van Delft MF, Glaser SP, Takiguchi M, Strasser A, Bouillet P, Adams JM, Huang DC
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 7;111(1):261-6
Proceedings of the National Academy of Sciences of the United States of America 2014 Jan 7;111(1):261-6
IL-15 regulates Bcl-2 family members Bim and Mcl-1 through JAK/STAT and PI3K/AKT pathways in T cells.
Shenoy AR, Kirschnek S, Häcker G
European journal of immunology 2014 Aug;44(8):2500-7
European journal of immunology 2014 Aug;44(8):2500-7
Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1.
Maurer U, Charvet C, Wagman AS, Dejardin E, Green DR
Molecular cell 2006 Mar 17;21(6):749-60
Molecular cell 2006 Mar 17;21(6):749-60
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 5 µg/mL of Anti-Human/Mouse MCL-1 Purified, followed by Anti-Mouse IgG Biotin, streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
