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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- 14-6701-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCL-1 Monoclonal Antibody (Ab22), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This Ab22 monoclonal antibody reacts with the human myeloid cell leukemia-1 (Mcl-1) protein. This 40 kDa Bcl-2 family member is expressed in B and T cells, macrophages, neutrophils, and hematopoietic stem cells. Mcl-1 is also expressed in non-immune cells such as fibroblasts, epithelial cells, neuroendocrine cells, chondrocytes, and hepatocytes. Reportedly induced by growth factors and cytokines in normal cells, Mcl-1 expression of Mcl-1 can be dysregulated in various leukemias and cancer. Mcl-1 mediates cell survival and proliferation by sequestering the pro-apoptotic Bcl-2 proteins Bak, Bax, and Bim. This protein is also involved in germinal center and memory B cell formation, as well as in myeloid and early lymphoid differentiation. Finally, Mcl-1 activity and dimerization with Bcl-2 family members is dependent on serine and threonine phosphorylation.
- Antibody clone number
- Ab22
- Concentration
- 0.5 mg/mL
Submitted references Mcl-1; the molecular regulation of protein function.
Thomas LW, Lam C, Edwards SW
FEBS letters 2010 Jul 16;584(14):2981-9
FEBS letters 2010 Jul 16;584(14):2981-9
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- Experimental details
- Immunocytochemistry of fixed and permeabilized MCF-7 cells using 5 µg/mL of Mouse IgG1 kappa Isotype Control (Product # 14-4714-82) (left) or Anti-Human Mcl-1 Purified (right) followed by Anti-Mouse TRITC. Nuclei are counterstained with DAPI.
