Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-64688 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MCL-1 (Thr163) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Phospho-MCL1 (Thr163) Polyclonal Antibody detects endogenous levels of MCL1 only when phosphorylated at Thr163.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references An FBXW7-ZEB2 axis links EMT and tumour microenvironment to promote colorectal cancer stem cells and chemoresistance.
Li N, Babaei-Jadidi R, Lorenzi F, Spencer-Dene B, Clarke P, Domingo E, Tulchinsky E, Vries RGJ, Kerr D, Pan Y, He Y, Bates DO, Tomlinson I, Clevers H, Nateri AS
Oncogenesis 2019 Feb 19;8(3):13
Oncogenesis 2019 Feb 19;8(3):13
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCL1 in PMA treated A549 whole cell lysates using a Phospho-MCL1 (Thr163) Polyclonal Antibody (Product # PA5-64688).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-MCL-1 (Thr163) in paraffin-embedded human Squamous cell carcinoma of lung. 1) Phospho-MCL-1 (Thr163) Polyclonal Antibody (Product # PA5-64688) was diluted at 1:200 (4° overnight). 2) Tris-EDTA, pH9.0 was used for antigen retrieval. 3) Secondary antibody was diluted at 1:200 (room temperature, 45 min).