TA500989

antibody from OriGene

Targeting: MCL1 BCL2L3, Mcl-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500989

Provider

OriGene

Proper citation

OriGene Cat#TA500989, RRID:AB_11128794

Product name

Anti-MCL1 mouse monoclonal antibody, clone OTI10C5 (formerly 10C5)

Antibody type

Monoclonal

Description

Anti-MCL1 mouse monoclonal antibody, clone OTI10C5 (formerly 10C5)

Host

Mouse

Conjugate

Unconjugated

Epitope

MCL1

Isotype

IgG

Antibody clone number

OTI10C5

Vial size

100 µl

Concentration

0.75 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse tissue by using anti-MCL1 monoclonal antibody. (1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-MCL1 mouse monoclonal antibody (TA500989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1(RC200521).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500989, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500989, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500989, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-MCL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500989, Dilution 1:50)

Validation comment

IHC