M00712

antibody from Boster Biological Technology

Targeting: MCL1 BCL2L3, Mcl-1

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00712

Provider

Boster Biological Technology

Product name

Anti-MCL1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for Mcl-1/MCL1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat Mcl-1/MCL1 information: Molecular Weight: 37337 MW; Subcellular Localization: Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

BCO-13

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Systematic and experimental investigations of the anti-colorectal cancer mediated by genistein.

Zhang L, Zhang J, Gong Y, Lv L
BioFactors (Oxford, England) 2020 Nov;46(6):974-982

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human stomach, using MCL1 Antibody(M00712)MCL1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MCL1 Antibody (M00712)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image