Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-15236 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCL-1 Monoclonal Antibody (8C6, 8C6D4B1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15236 targets MCL-1 in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15236 immunogen is purified recombinant fragment of human MCL-1 expressed in E. Coli.. MA5-15236 detects MCL-1 which has a predicted molecular weight of approximately 37kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8C6, 8C6D4B1
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The role of microRNA expression pattern in human intrahepatic cholangiocarcinoma.
Chen L, Yan HX, Yang W, Hu L, Yu LX, Liu Q, Li L, Huang DD, Ding J, Shen F, Zhou WP, Wu MC, Wang HY
Journal of hepatology 2009 Feb;50(2):358-69
Journal of hepatology 2009 Feb;50(2):358-69
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCL-1 using MCL-1 monoclonal antibody (Product # MA5-15236) in HeLa (1), BCBL-1 (2), Jurkat (3) and HL60 (4) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HepG2 cells using MCL-1 monoclonal antibody (Product # MA5-15236) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lymph node tissues using MCL-1 monoclonal antibody (Product # MA5-15236) followed with DAB staining.