PA5-48060

antibody from Invitrogen Antibodies

Targeting: SOCS5 CIS6, Cish5, CISH6, KIAA0671, SOCS-5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-48060

Provider

Invitrogen Antibodies

Product name

SOCS5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant human SOCS-1, -2, -3, -6, and -7 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-48060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for SOCS-5 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SOCS5 in HeLa human cervical epithelial carcinoma cell line. Samples were incubated in SOCS5 polyclonal antibody (Product # PA5-48060) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for SOCS‚5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SOCS5 in immersion fixed Jurkat human acute T cell leukemia cell line. Samples were incubated in SOCS5 polyclonal antibody (Product # PA5-48060) using a dilution of 15 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.