Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- A02359-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Nicotinic Acetylcholine Receptor alpha 5/CHRNA5 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for CHRNA5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CHRNA5 information: Molecular Weight: 53054 MW; Subcellular Localization: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.
Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth.
Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.
Sun Z, Zhangsun M, Dong S, Liu Y, Qian J, Zhangsun D, Luo S
Marine drugs 2020 Jan 17;18(1)
Marine drugs 2020 Jan 17;18(1)
Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth.
Qian J, Liu YQ, Sun ZH, Zhangsun DT, Luo SL
European journal of pharmacology 2019 Dec 15;865:172674
European journal of pharmacology 2019 Dec 15;865:172674
Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.
Liu Y, Qian J, Sun Z, Zhangsun D, Luo S
Marine drugs 2019 Apr 28;17(5)
Marine drugs 2019 Apr 28;17(5)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of CHRNA5 using anti-CHRNA5 antibody (A02359-2). CHRNA5 was detected in paraffin-embedded section of human prostatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-CHRNA5 Antibody (A02359-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image