Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-31687 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRNA5 Monoclonal Antibody (2D2D10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-31687 has been tested in indirect ELISA.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D2D10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHRNA5 in membrane protein lysate of C6 (1), membrane protein lysate of SK-N-SH (2), membrane protein lysate of C6 (3), and C6 (4) cell lysate. Samples were incubated with CHRNA5 monoclonal antibody (Product # MA5-31687) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHRNA5 in human CHRNA5 recombinant protein. Sample was incubated with CHRNA5 monoclonal antibody (Product # MA5-31687) using a dilution of 1:500-1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CHRNA5 in HEK293 (1) and CHRNA5 cell lysate. Samples were incubated with CHRNA5 monoclonal antibody (Product # MA5-31687) using a dilution of 1:500-1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of CHRNA5 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with CHRNA5 monoclonal antibody (Product # MA5-31687) using a dilution of 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CHRNA5 in SK-N-SH cells (green). Sample was incubated with CHRNA5 monoclonal antibody (Product # MA5-31687) using a dilution of 1:200-1:400 followed by negative control (red).
