H00002770-M01

antibody from Abnova Corporation

Targeting: GNAI1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002770-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002770-M01, RRID:AB_425453

Product name

GNAI1 monoclonal antibody (M01), clone 2B8-2A5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant GNAI1.

Antigen sequence

MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVK
LLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYK
AVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDAR
QLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACF
NRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVL
RTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKK
WIHCFEGVAAIIFCVALSDYDLVLAEDEEMNRMHE
SMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKK
SPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRKD
TKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKD
CGLF


Isotype

IgG

Antibody clone number

2B8-2A5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

GNAI1 monoclonal antibody (M01), clone 2B8-2A5. Western Blot analysis of GNAI1 expression in human liver.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.Lane 1: GNAI1 transfected lysate(40.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GNAI1 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol