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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-28223 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAI1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLaS3.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of G protein alpha inhibitor 1 using 30 µg of A) A431 and B) HeLa S3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a G protein alpha inhibitor 1 polyclonal antibody (Product # PA5-28223) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GNAI1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GNAI1 Polyclonal Antibody (Product # PA5-28223) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GNAI1 Polyclonal Antibody detects GNAI1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GNAI1 Polyclonal Antibody (Product # PA5-28223) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
