HPA008689

CPA3 antibody from Atlas Antibodies

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Enhanced validation

Provider product page for HPA008689

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Antibody Data

Product number

HPA008689

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA008689, RRID:AB_1233677

Product name

Anti-CPA3

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KNQNSKCIGTDLNRNFNASWNSIPNTNDPCADNYR
GSAPESEKETKAVTNFIRSHLNEIKVYITFHSYSQ
MLLFPYGYTSKLPPNHEDLAKVAKIGTDVLSTRYE
TRYIYGPIESTIYPISGSSL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

References

Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps
Takabayashi T, Kato A, Peters A, Suh L, Carter R, Norton J, Grammer L, Tan B, Chandra R, Conley D, Kern R, Fujieda S, Schleimer R
Journal of Allergy and Clinical Immunology 2012 August;130(2):410-420

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419698)

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human nasopharynx shows distinct positivity in subsets of inflammatory cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human rectum and skeletal muscle tissues using Anti-CPA3 antibody. Corresponding CPA3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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