- PA1-929 - Invitrogen Antibodies KCNQ2 antibody

Submitted references Flupirtine enhances NHE-3-mediated Na(+) absorption in rat colon via an ENS-dependent mechanism.

Nickerson AJ, Rajendran VM
American journal of physiology. Gastrointestinal and liver physiology 2021 Aug 1;321(2):G185-G199

The M-current works in tandem with the persistent sodium current to set the speed of locomotion.

Verneuil J, Brocard C, Trouplin V, Villard L, Peyronnet-Roux J, Brocard F
PLoS biology 2020 Nov;18(11):e3000738

FGF14 is a regulator of KCNQ2/3 channels.

Pablo JL, Pitt GS
Proceedings of the National Academy of Sciences of the United States of America 2017 Jan 3;114(1):154-159

Giant ankyrin-G: a critical innovation in vertebrate evolution of fast and integrated neuronal signaling.

Jenkins PM, Kim N, Jones SL, Tseng WC, Svitkina TM, Yin HH, Bennett V
Proceedings of the National Academy of Sciences of the United States of America 2015 Jan 27;112(4):957-64

Requirement of subunit co-assembly and ankyrin-G for M-channel localization at the axon initial segment.

Rasmussen HB, Frøkjaer-Jensen C, Jensen CS, Jensen HS, Jørgensen NK, Misonou H, Trimmer JS, Olesen SP, Schmitt N
Journal of cell science 2007 Mar 15;120(Pt 6):953-63

Antibodies and a cysteine-modifying reagent show correspondence of M current in neurons to KCNQ2 and KCNQ3 K+ channels.

Roche JP, Westenbroek R, Sorom AJ, Hille B, Mackie K, Shapiro MS
British journal of pharmacology 2002 Dec;137(8):1173-86

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of KCNQ2 using Anti-KCNQ2 Polyclonal Antibody (Product # PA1-929) shows staining in C6 Cells. KCNQ2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing KCNQ2 (Product # PA1-929) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of KCNQ2 using Anti-KCNQ2 Polyclonal Antibody (Product # PA1-929) shows staining in HepG2 Cells. KCNQ2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing KCNQ2 (Product # PA1-929) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of KCNQ2 using Anti-KCNQ2 Polyclonal Antibody (Product # PA1-929) shows staining in U251 Cells. KCNQ2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing KCNQ2 (Product # PA1-929) at a dilution of 1:20 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552, Goat Anti-Rabbit). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 Lumbar interneurons within the locomotor CPG region ubiquitously express Kv7.2-containing channels. (A-F) Immunostaining of lumbar (L1-L2) ventromedial interneurons from juvenile rats ( n = 3 rats) against Kv7.2 (A, n = 216 cells) or Kv7.3 (D, n = 170 cells) along the AIS labeled by the pan-Na v antibody (B,E). Kv7.2 and pan-Na v are merged in (C), and Kv7.3 and pan-Na v are merged in (F). Asterisks indicate the nucleus position and arrowheads the AIS. Scale bars = 10 mum. (G) Group means quantification of the proportion of pan-Na v positive interneurons expressing Kv7.2 or Kv7.3 channels. (H) Group means quantification of the start and end positions of pan-Na v and Kv7.2 immunolabeling along the axonal process from the soma ( n = 10 cells). *** P < 0.001, comparing start or end positions between groups; Mann-Whitney test. Data are mean +- SEM. Underlying numerical values can be found in the S1 Data . AIS, axonal initial segment; CPG, central pattern generator; Na v , voltage-gated sodium channel.

PA1-929

Antibody data