PA5-109878

antibody from Invitrogen Antibodies

Targeting: KIF17 KIAA1405, KIF17B, KIF3X, KLP-2, OSM-3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-109878

Provider

Invitrogen Antibodies

Product name

KIF17 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LKAILTQQMS PSSLSALLSR QVPPDPVQVE EKLLPQPVIQ HDVEAEKQLI REEYEERLAR LKADYKAEQE SRARLEEDIT AMRNSYDVRL STLEENLRKE TEAVLQVGVL YKAEVMSRAE FASSAEYPPA FQYETVVKPK VFSTTDTLPS DDVSKTQVSS RFAELPKVEP SKSEISLGSS ESSSLEETSV SEAFPGPEEP SNVEVSMPTE ESRSRYFLDE CLGQEAAGHL LGEQNYLPQE EPQEVPLQGL LGLQDPFAEV EAKLARLSST VARTDAPQAD VPKVPVQVPA PTDLLEPSDA R

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.28 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KIF17 in extracts of various cell lines. Samples were incubated with KIF17 Polyclonal antibody (Product # PA5-109878) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KIF17 in rat brain. Samples were incubated in polyclonal KIF17 antibody (Product # PA5-109878) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KIF17 in rat brain. Samples were incubated in polyclonal KIF17 antibody (Product # PA5-109878) using a dilution of 1:100, followed by DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of KIF17 in rat brain. Samples were incubated in polyclonal KIF17 antibody (Product # PA5-109878) using a dilution of 1:100, followed by DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KIF17 in paraffin-embedded human lung cancer. Samples were incubated with KIF17 Polyclonal antibody (Product # PA5-109878) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KIF17 in paraffin-embedded rat testis. Samples were incubated with KIF17 Polyclonal antibody (Product # PA5-109878) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Immunofluorescence) analysis of KIF17 in rat brain. Samples were incubated with KIF17 Polyclonal antibody (Product # PA5-109878) using a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KIF17 in paraffin-embedded rat testis. Samples were incubated with KIF17 Polyclonal antibody (Product # PA5-109878) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.