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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- PA1-16666 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- 15-PGDH Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- Suggested positive control: antigen standard for HPGD (transient overexpression lysate), Treated A549 lung carcinoma cell lysates and LoVo colon carcinoma cell lysates.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of 15-PGDH in LoVo whole cell lysates using Product # PA1-16666. Lane 1 uses a 1:6,000 dilution. Lane 2 uses a 1:1, 500 dilution. ECL: 10 second exposure.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 15-PGDH in 0.5 mg/mL Lovo (left) and HeLa (right) lysate. Samples were incubated in 15-PGDH polyclonal antibody (Product # PA1-16666). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 15-PGDH in Lovo cell lysate. Sample was incubated in 15-PGDH polyclonal antibody (Product # PA1-16666).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of 15-PGDH in 0.5 mg/mL Lovo (left) and HeLa (right) lysate. Samples were incubated in 15-PGDH polyclonal antibody (Product # PA1-16666). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of 15-PGDH in HeLa cells. Samples were incubated in 15-PGDH polyclonal antibody (Product # PA1-16666) followed by DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
