HPA004919

HPGD antibody from Atlas Antibodies

SDR36C1

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Enhanced validation

Immunohistochemistry

Enhanced validation

Provider product page for HPA004919

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Antibody Data

Product number

HPA004919

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA004919, RRID:AB_887574

Product name

Anti-HPGD

Provider product page

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQ
QLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTL
QINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSL
AGLMPVAQQPV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Characterization of Transcriptional Changes in ERG Rearrangement-Positive Prostate Cancer Identifies the Regulation of Metabolic Sensors Such as Neuropeptide Y
Massoner P, Kugler K, Unterberger K, Kuner R, Mueller L, Fälth M, Schäfer G, Seifarth C, Ecker S, Verdorfer I, Graber A, Sültmann H, Klocker H, Koul H
PLoS ONE 2013 February;8(2)

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Candidate Serological Biomarkers for Cancer Identified from the Secretomes of 23 Cancer Cell Lines and the Human Protein Atlas
Wu C, Hsu C, Chen C, Yu C, Chang K, Tai D, Liu H, Su W, Chang Y, Yu J
Molecular & Cellular Proteomics 2010 June;9(6):1100-1117

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HEL.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line RT-4 and human cell line U-251 MG.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Western blot analysis using Anti-HPGD antibody HPA004919 (A) shows similar pattern to independent antibody HPA005679 (B).

Sample type

HUMAN

Antibody #2 product nr

HPA005679

Antibody provider

Atlas Antibodies

Independent Antibody Method

WB

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human urinary bladder and skeletal muscle tissues using Anti-HPGD antibody. Corresponding HPGD RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more