Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB46361-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human GPR56 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human GPR56 in direct ELISAs.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q9Y653- Isotype
- IgG
- Antibody clone number
- 2446B
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human GPR56 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human GPR56. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human GPR56 Monoclonal Antibody (Catalog # MAB46361) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for GPR56 at approximately 75 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control.This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- GPR56 in T47D human breast cancer cell line. GPR56 was detected in immersion fixed T47D human breast cancer cell line (left panel; positive staining) and WM-115 human malignant melanoma cell line (right panel; negative staining) using Rabbit Anti-Human GPR56 Monoclonal Antibody (Catalog # MAB46361) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of GPR56 in Human Peripheral Blood Cells by Flow Cytometry. Human peripheral blood cells were stained with (A) Rabbit Anti-Human GPR56 Monoclonal Antibody (Catalog # MAB46361) or (B) Rabbit IgG isotype control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and Mouse anti-Human CD56 PE-conjugated monoclonal antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.
