PA5-52323

antibody from Invitrogen Antibodies

Targeting: NLGN1 KIAA1070

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52323

Provider

Invitrogen Antibodies

Product name

Neuroligin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: DQLYLHIGLK PRVKEHYRAN KVNLWLELVP HLHNLNDISQ YTSTTTKVPS TDITFRPTRK NSVPVTSAFP TAKQDDPKQQ PSPFSVDQRD YS

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Neuroligin 1 in control (vector only transfected HEK293T lysate) and NLGN1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Neuroligin 1 Polyclonal Antibody (Product # PA5-52323).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot was performed using Anti-Neuroligin 1 Polyclonal Antibody (Product # PA5-52323) and a 110 kDa band corresponding to Neuroligin-1 was observed only in Mouse Brain, Rat Brain and Mouse Cerebellum which are reported to be positive and not in any other tissues like Mouse Mammary gland and Skeletal muscle. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Mouse Mammary gland (Lane 4) and Mouse Skeletal Muscle (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).An uncharacterized band of ~25 kDa and 35 kDa were also observed across the tissues.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of NLGN1 in human cerebral cortex tissue shows strong cytoplasmic positivity in neuronal cells. Samples were probed using a NLGN1 Polyclonal Antibody (Product # PA5-52323).