STJ99111
antibody from St John's Laboratory
Targeting: CRTC1
FLJ14027, KIAA0616, MECT1, TORC1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
Reference
Comment
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- Product number
- STJ99111 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-CRTC1 antibody [3D7-E5-D9] (STJ99111)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-Creb-Regulated Transcription Coactivator 1 is suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunoprecipitation research applications.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA
- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Western blot detection of MECT1/Torc1 in Hela and Jurkat lysates using MECT1/Torc1 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 78KDa.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunocytochemistry stain of Hela using MECT1/Torc1 mouse mAb (1:300).
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Hela cell lysate using MECT1/Torc1 mouse mAb.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of K562 cells stained with TORC1 (N-terminus) (red, 1/100 dilution) , followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA