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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-77940 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77940, RRID:AB_11010117
- Product name
- Rabbit Polyclonal TORC1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against human MECT1 protein. A BLAST analysis was used to suggest cross reactivity with MECT1 protein from human, mouse, rat, zebrafish, pufferfish and bovine based on 100% identity with the immunizing sequence.
- Reactivity
- Human, Mouse, Rat, Bovine, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ECT2 Antibody [NBP1-77940] - Shows detection of MECT2 (lower arrowhead) and MECT2:MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing MECT2 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing MECT2 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Frederick Kaye, CCR-NCI, Bethesda, MD.
