NB100-2352

antibody from Novus Biologicals

Targeting: CRTC1 FLJ14027, KIAA0616, MECT1, TORC1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2352

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2352, RRID:AB_2085926

Product name

Rabbit Polyclonal TORC1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TORC1 Antibody [NB100-2352] - Detection of Human and Mouse TORC1 on HeLa whole cell lysate using NB100-2352. TORC1 was also immunoprecipitated by another rabbit anti-TORC1 antibody and NB100-2351.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TORC1 Antibody [NB100-2352] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TORC1 Antibody [NB100-2352] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TORC1 Antibody [NB100-2352] - Detection of human TORC1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TORC1 antibody NB100-2352 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TORC1 Antibody [NB100-2352] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TORC1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: TORC1 Antibody [NB100-2352] - Detection of human TORC1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TORC1 used at a dilution of 1:200 (1ug/ml). Detection: DAB