PA3-813

antibody from Invitrogen Antibodies

Targeting: RARG NR1B3, RARC

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-813

Provider

Invitrogen Antibodies

Product name

RAR gamma-1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA3-813 detects recombinant human retinoic acid receptor (RAR) gamma-1. The immunizing sequence is 100% conserved in mouse. This antibody detects RAR gamma-1, but not RAR gamma-2, or either of the alpha or beta forms of RAR.

Concentration

Conc. Not Determined

References

Submitted references

Pharmacologic retinoid signaling and physiologic retinoic acid receptor signaling inhibit basal cell carcinoma tumorigenesis.

So PL, Fujimoto MA, Epstein EH Jr
Molecular cancer therapeutics 2008 May;7(5):1275-84

---

The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.

Rioux L, Arnold SE
Psychiatry research 2005 Jan 30;133(1):13-21

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB detection of RAR gamma 1 from human HEK293 cells. Lane 1 shows PA3-813 incubated with the neutralizing peptide (PEP-228), lane 2 shows RAR detection by PA3-813.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extract (30 µg lysate) of Rat Brain (Lane 1). The blot was probed with Anti-RARG Rabbit Polyclonal Antibody (Product # PA3-813, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to RARG was observed in the tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extract (30 µg lysate) of Rat Brain (Lane 1). The blot was probed with Anti-RARG Rabbit Polyclonal Antibody (Product # PA3-813, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to RARG was observed in the tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-RAR gamma-1 Polyclonal Antibody (Product # PA3-813) and a 60 kDa band corresponding to RAR gamma-1 was observed in MCF-7, HaCaT and Mouse Mammary Gland but not in BeWo and HepG2. Nuclear enriched extracts (50 µg lysate) of MCF7 (Lane 1), BeWo (Lane 2), HaCaT (Lane 3), Hep G2 (Lane 4) and tissue extract (50 µg lysate) of Mouse Mammary gland (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).