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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00008672-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008672-M03, RRID:AB_606173
- Product name
- EIF4G3 monoclonal antibody (M03), clone 1H5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant EIF4G3.
- Antigen sequence
MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATI
PNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYP
VPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGP
FYPGPGPGDFPNAYGTPFYPSQPVYQSAPIMVPTQ
QQPPPAKREKKTIRIRDPNQGGKDITEEIISGGGS
RNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTV
ESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPV
LRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPT
TVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELS
SPREDTIPIPSLTSCTETSDPLPTNENDGDICKKP
CSVAPNDIPLVSSTNLINEINGVSEKLSATESIVE
IVKQEVLPLTLELEILENPPEEMKLECIPAPITPS
TVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSA
AITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADG
QTEEILDSQNLNSRRSPVPETSNEC- Isotype
- IgG
- Antibody clone number
- 1H5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- EIF4G3 monoclonal antibody (M03), clone 1H5. Western Blot analysis of EIF4G3 expression in HeLa ( Cat # L013V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of EIF4G3 expression in transfected 293T cell line by EIF4G3 monoclonal antibody (M03), clone 1H5.Lane 1: EIF4G3 transfected lysate(55.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged EIF4G3 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
