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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00008672-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008672-M03, RRID:AB_538517
- Product name
- Mouse Monoclonal eIF4GII Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EIF4G3 - eukaryotic translation initiation factor 4 gamma, 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - EIF4G3 over-expressed 293 cell line, cotransfected with EIF4G3 Validated Chimera RNAi or non-transfected control. Blot probed with H00008672-M03. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of EIF4G3 expression in transfected 293T cell line by EIF4G3 monoclonal antibody (M03), clone 1H5. Lane 1: EIF4G3 transfected lysatE (55.2 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of EIF4G3 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: eIF4GII Antibody (1H5) [H00008672-M03] - Detection limit for recombinant GST tagged EIF4G3 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml
