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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Other assay [3]
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- Product number
- PA5-31101 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF4G3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, mouse testis.
- Concentration
- 0.26 mg/mL
Submitted references eIF4E3 forms an active eIF4F complex during stresses (eIF4FS) targeting mTOR and re-programs the translatome.
Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.
Weiss B, Allen GE, Kloehn J, Abid K, Jaquier-Gubler P, Curran JA
Nucleic acids research 2021 May 21;49(9):5159-5176
Nucleic acids research 2021 May 21;49(9):5159-5176
Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.
Hu J, Sun F, Handel MA
Biology of reproduction 2018 Jan 1;98(1):102-114
Biology of reproduction 2018 Jan 1;98(1):102-114
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4G3 using 30 µg of A) 293T (B) A431 (C) HeLa and D) HepG2 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an EIF4G3 polyclonal antibody (Product # PA5-31101) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EIF4G3 using 30 µg of A) JC and B) BCL-1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an EIF4G3 polyclonal antibody (Product # PA5-31101) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eIF4G3 was performed by separating 50 µg of mouse tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a eIF4G3 Polyclonal Antibody (Product # PA5-31101) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of eIF4G3 was performed by separating 30 µg of various whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a eIF4G3 Polyclonal Antibody (Product # PA5-31101) at a dilution of 1:10000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of eIF4G3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF4G3 Polyclonal Antibody (Product # PA5-31101) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF4G3 was performed in paraffin-embedded mouse brain tissue using eIF4G3 Polyclonal Antibody (Product # PA5-31101) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF4G3 was performed in paraffin-embedded mouse testis tissue using eIF4G3 Polyclonal Antibody (Product # PA5-31101) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF4G3 was performed in paraffin-embedded rat brain tissue using eIF4G3 Polyclonal Antibody (Product # PA5-31101) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of eIF4G3 was performed in frozen-sectioned adult mouse cerebellum tissue using eIF4G3 Polyclonal Antibody (Product # PA5-31101) at a dilution of 1:250 (Green). Red: NeuN, stained by NeuN antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF4G3 Polyclonal Antibody detects EIF4G3 protein at cytosol on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain stem. EIF4G3 Polyclonal Antibody (Product # PA5-31101) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF4G3 Polyclonal Antibody detects EIF4G3 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. EIF4G3 Polyclonal Antibody (Product # PA5-31101) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- eIF4G3 Polyclonal Antibody detects EIF4G3 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. EIF4G3 Polyclonal Antibody (Product # PA5-31101) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of EIF4G3 was performed in 293T whole cell extracts using 5 µg of eIF4G3 Polyclonal Antibody (Product # PA5-31101). Samples were transferred to a membrane and probed with eIF4G3 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. The eIF4E3 partners discovered by Y2H. ( A ) Western blot analysis of the co-IP assay using eIF4E3 HA and endogenous eIF4G1 or eIF4G3 starting from a lysate of HEK293T cells transduced with eIF4E3 HA treated with DMSO or with 250 nM Torin1 for 2 h. Beads carrying covalently cross-linked Anti-HA Ab (+IgG) were used to immunoprecipitate eIF4E3 HA . Beads without Ab served as a control (-IgG). (B-H) Bimolecular complementation assay using Venus YFP in transfected HEK293T cells. Images were generated by confocal microscopy. Venus fragment 1 and Venus fragment 2 were fused to eIF4E3 and to one of the tested partners, respectively: empty vector ( B ), eIF4G1 ( C ), eIF4G3 ( D ), HSPA8 ( E ), XIAP ( F ), HIF1AN ( G ) and CDC5L ( H ).
