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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- LS-B721 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B721, RRID:AB_2190003
- Product name
- IHC-plus™ SLIT1 Antibody LS-B721
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-SLIT-1 Antibody - Western Blot. Western blot of Affinity Purified anti-SLIT-1 antibody shows detection of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates. The expected molecular weight for SLIT-1 is 168 kD. Approximately 20 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40kD -160kD (see Little et al, 2002 (Int J Dev Biol. 2002;46(4):385-91) for additional details regarding SLIT1 alternative splicing).
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-SLIT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-SLIT1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
