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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB11326 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11326, RRID:AB_1714203
- Product name
- Slit1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Slit1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Multiple roles for slits in the control of cell migration in the rostral migratory stream.
Conserved roles for Slit and Robo proteins in midline commissural axon guidance.
On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion.
Nguyen-Ba-Charvet KT, Picard-Riera N, Tessier-Lavigne M, Baron-Van Evercooren A, Sotelo C, Chédotal A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Feb 11;24(6):1497-506
The Journal of neuroscience : the official journal of the Society for Neuroscience 2004 Feb 11;24(6):1497-506
Conserved roles for Slit and Robo proteins in midline commissural axon guidance.
Long H, Sabatier C, Ma L, Plump A, Yuan W, Ornitz DM, Tamada A, Murakami F, Goodman CS, Tessier-Lavigne M
Neuron 2004 Apr 22;42(2):213-23
Neuron 2004 Apr 22;42(2):213-23
On the topographic targeting of basal vomeronasal axons through Slit-mediated chemorepulsion.
Knöll B, Schmidt H, Andrews W, Guthrie S, Pini A, Sundaresan V, Drescher U
Development (Cambridge, England) 2003 Nov;130(21):5073-82
Development (Cambridge, England) 2003 Nov;130(21):5073-82
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Supportive validation
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- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using Slit1 polyclonal antibody (Cat # PAB11326) shows detection of Slit1 in rat (Lane 1) and mouse (Lane 2) brain lysates.The expected molecular weight for Slit1 is 168 KDa.Approximately 20 &micr;g of each lysates was run on a SDS-PAGE and reaction with a 1 : 500 dilution of Slit1 polyclonal antibody.Note: The smaller strong bands observed in this blot are likely Slit1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from ~40 KDa -160 KDa (see Little et al, 2002 for additional details).
