AF3134

antibody from Novus Biologicals

Targeting: COMP EDM1, EPD1, MED, PSACH, THBS5

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3134

Provider

Novus Biologicals

Product name

Goat Polyclonal COMP/Thrombospondin-5 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human COMP in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Thrombospondin-1, -2, -3 and -4 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP.

Sun L, Wang Y, Wang L, Yao B, Chen T, Li Q, Liu Z, Liu R, Niu Y, Song T, Liu Q, Tu K
Journal of experimental & clinical cancer research : CR 2019 Apr 18;38(1):170

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Cartilage oligomeric matrix protein is differentially expressed in human subcutaneous adipose tissue and regulates adipogenesis.

Denton N, Pinnick KE, Karpe F
Molecular metabolism 2018 Oct;16:172-179

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human COMP/Thrombospondin-5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human COMP/Thrombospondin-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for COMP/Thrombospondin-5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Adhesion Mediated by COMP/Thrombospondin-5 and Neutralization by Human COMP/Thrombospondin-5 Antibody. Recombinant Human COMP/Thrombospondin-5 (Catalog # 3134-CP), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human COMP/Thrombospondin-5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human COMP/Thrombo-spondin-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134). The ND₅₀ is typically 1-4 µg/mL.