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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- AF3134 - Provider product page
- Provider
- R&D Systems
- Product name
- Human COMP/Thrombospondin-5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human COMP in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Thrombospondin-1, -2, -3 and -4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P49747- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP.
Cartilage oligomeric matrix protein is differentially expressed in human subcutaneous adipose tissue and regulates adipogenesis.
Sun L, Wang Y, Wang L, Yao B, Chen T, Li Q, Liu Z, Liu R, Niu Y, Song T, Liu Q, Tu K
Journal of experimental & clinical cancer research : CR 2019 Apr 18;38(1):170
Journal of experimental & clinical cancer research : CR 2019 Apr 18;38(1):170
Cartilage oligomeric matrix protein is differentially expressed in human subcutaneous adipose tissue and regulates adipogenesis.
Denton N, Pinnick KE, Karpe F
Molecular metabolism 2018 Oct;16:172-179
Molecular metabolism 2018 Oct;16:172-179
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human COMP/Thrombospondin-5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human COMP/Thrombospondin-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for COMP/Thrombospondin-5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by COMP/Thrombospondin-5 and Neutralization by Human COMP/Thrombospondin-5 Antibody. Recombinant Human COMP/Thrombospondin-5 (Catalog # 3134-CP), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human COMP/Thrombospondin-5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human COMP/Thrombo-spondin-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134). The ND50 is typically 1-4 µg/mL.
