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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA1-31987 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ATAD5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-31987 detects Elg 1 from human samples.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot using ATAD5 Polyclonal Antibody (Product # PA1-31987) shows detection of a band 120 kDa corresponding to human Elg1 in various cell lysates. Lanes contain 5µg of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjµgated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, althoµgh the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).
