Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09255PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09255PU-N, RRID:AB_2035455
- Product name
- anti ATAD5 (63-76)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.50 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~ 5 μg of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).