Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27691 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERCA3 ATPase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, K562, BCL-1. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (92%), Chicken (88%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SERCA3 using 30 µg of A) Jurkat (B) Raji and C) K562 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a SERCA3 polyclonal antibody (Product # PA5-27691) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SERCA3 ATPase Polyclonal Antibody (Product # PA5-27691). Sample (30 µg of whole cell lysate). Lane A: BCL-1 . 5% SDS PAGE. SERCA3 ATPase Polyclonal Antibody (Product # PA5-27691) diluted at 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SERCA3 ATPase Polyclonal Antibody (Product # PA5-27691). Jurkat whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SERCA3 ATPase Polyclonal Antibody (Product # PA5-27691) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SERCA3 (Product # PA5-27691) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.