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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA5-54457 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF135 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSILGKAFSS GVDLSMASPK LVTSDTAAGK IRDILHDLEE IQEKLQESVT WKEAPEAQMQ GELLEAPSSS SCPLPDQSHP ALRRASRFAQ WAIHPTFNLK Highest antigen sequence identity to the following orthologs: Mouse - 46%, Rat - 49%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references RNF135 Promoter Methylation Is Associated With Immune Infiltration and Prognosis in Hepatocellular Carcinoma.
Wang X, Chen M, Liang X, Bai Y, Zeng J, Xu X, Li H, Wang J, Fan K, Zhao G
Frontiers in oncology 2021;11:752511
Frontiers in oncology 2021;11:752511
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 The effects of RNF135 expression on cell migration and invasion. (A) RNF135 related pathways in HCC patients were analyzed by GSEA. (B) Knockdown and overexpression efficiency of RNF135 in cell lines was measured by western blot. (C, D) Wound healing assay was used to detect cell migration (up), and Student's t-test for unpaired was applied between experimental group and control group (down). (E, F) Transwell assay was used to evaluate cell invasion (up), and Student's t-test for unpaired was applied between experimental group and control group (down). All experiments were repeated three times, and data were expressed as mean +- SD. * P < 0.05.
