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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-54457 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF135 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSILGKAFSS GVDLSMASPK LVTSDTAAGK IRDILHDLEE IQEKLQESVT WKEAPEAQMQ GELLEAPSSS SCPLPDQSHP ALRRASRFAQ WAIHPTFNLK
- Concentration
- 0.2 mg/mL
Submitted references RNF135 Promoter Methylation Is Associated With Immune Infiltration and Prognosis in Hepatocellular Carcinoma.
Wang X, Chen M, Liang X, Bai Y, Zeng J, Xu X, Li H, Wang J, Fan K, Zhao G
Frontiers in oncology 2021;11:752511
Frontiers in oncology 2021;11:752511
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF135 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4. Samples were probed using a RNF135 Polyclonal Antibody (Product # PA5-54457).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF135 in human esophagus using a RNF135 Polyclonal Antibody (Product # PA5-54457) shows cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF135 in human liver using RNF135 Polyclonal Antibody (Product # PA5-54457) shows low positivity in hepatocytes as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF135 in human skin using RNF135 Polyclonal Antibody (Product # PA5-54457) shows moderate cytoplasmic positivity in squamous epithelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF135 in human prostate using RNF135 Polyclonal Antibody (Product # PA5-54457) shows moderate cytoplasmic positivity in smooth muscle cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 The effects of RNF135 expression on cell migration and invasion. (A) RNF135 related pathways in HCC patients were analyzed by GSEA. (B) Knockdown and overexpression efficiency of RNF135 in cell lines was measured by western blot. (C, D) Wound healing assay was used to detect cell migration (up), and Student's t-test for unpaired was applied between experimental group and control group (down). (E, F) Transwell assay was used to evaluate cell invasion (up), and Student's t-test for unpaired was applied between experimental group and control group (down). All experiments were repeated three times, and data were expressed as mean +- SD. * P < 0.05.
