25061-1-AP

antibody from Invitrogen Antibodies

Targeting: RNF135 MGC13061

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25061-1-AP

Provider

Invitrogen Antibodies

Product name

RNF135 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAGLGLGSA VPVWLAEDDL GCIICQGLLD WPATLPCGHS FCRHCLEALW GARDARRWAC PTCRQGAAQQ PHLRKNTLLQ DLADKYRRAA REIQAGSDPA HCPCPGSSSL SSAAARPRRR PELQRVAVEK SITEVAQELT ELVEHLVDIV RSLQNQRPLS ESGPDNELSI LGKENSWKPR LPPHAHCLTR ATLHSGELLG LLSGPSIQPL T (1-210 aa encoded by BC005084)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.45 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25061-1-AP ( RNF135 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HEK-293 cells using 25061-1-AP (RNF135 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25061-1-AP ( RNF135 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 25061-1-AP ( RNF135 Antibody) at dilution of 1:200 (under 40x lens).