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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09297PU-N - Provider product page
- Provider
- OriGene
- Product name
- MARK2 pThr595 rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- MARK2 pThr595 rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MARK2
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.6 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-MARK2 pT595 antibody shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead). HEK293 cells were transfected with pCMV-3xFlag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3xFlag-MARK2 only (lane 4). Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with anti-MARK2 pT595. An untransfected HEK293 cell lysate was used as a negative control (lane 2). MW markers are shown for size comparison (lane 1). PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phosphospecific staining are observed in lysates from cells transfected with both MARK2 and PKCzeta.
- Validation comment
- WB
