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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10332 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10332, RRID:AB_1707847
- Product name
- MARK2 (phospho T595) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of MARK2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Splicing alterations in human renal allografts: detection of a new splice variant of protein kinase Par1/Emk1 whose expression is associated with an increase of inflammation in protocol biopsies of transplanted patients.
Atypical PKC phosphorylates PAR-1 kinases to regulate localization and activity.
PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling.
Hueso M, Beltran V, Moreso F, Ciriero E, Fulladosa X, Grinyó JM, Serón D, Navarro E
Biochimica et biophysica acta 2004 May 24;1689(1):58-65
Biochimica et biophysica acta 2004 May 24;1689(1):58-65
Atypical PKC phosphorylates PAR-1 kinases to regulate localization and activity.
Hurov JB, Watkins JL, Piwnica-Worms H
Current biology : CB 2004 Apr 20;14(8):736-41
Current biology : CB 2004 Apr 20;14(8):736-41
PAR-1 is a Dishevelled-associated kinase and a positive regulator of Wnt signalling.
Sun TQ, Lu B, Feng JJ, Reinhard C, Jan YN, Fantl WJ, Williams LT
Nature cell biology 2001 Jul;3(7):628-36
Nature cell biology 2001 Jul;3(7):628-36
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Supportive validation
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- Experimental details
- Western blot using MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332) shows detection of a band at ~82 kDa corresponding to phosphorylated MARK2 (arrowhead).HEK293 cells were transfected with pCMV-3 x Flag-MARK2 plus pCMV-Flag-PKC-zeta (lane 3) or pCMV-3 x Flag-MARK2 only (lane 4).Total cell lysates were run in a 4-12% Nupage SDS-gel and probed with MARK2 (phospho T595) polyclonal antibody (Cat # PAB10332).An untransfected HEK293 cell lysate was used as a negative control (lane 2).MW markers are shown for size comparison (lane 1).PKC-zeta appears to be involved in the phosphorylation of T595 on MARK2 as increased amounts of phospho- specific staining are observed in lysates from cells transfected with both MARK2 and PKC-zeta.Personnel Communication, S. Shaw, NCI, Center for Cancer Research.
