Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00002011-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002011-M01, RRID:AB_2250358
- Product name
- Mouse Monoclonal MARK2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MARK2 - MAP/microtubule affinity-regulating kinase 2
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references MARK2/Par1b kinase present at centrosomes and retraction fibres corrects spindle off-centring induced by actin disassembly.
Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.
Pancreatic LKB1 deletion leads to acinar polarity defects and cystic neoplasms.
Hart M, Zulkipli I, Shrestha RL, Dang D, Conti D, Gul P, Kujawiak I, Draviam VM
Open biology 2019 Jun 28;9(6):180263
Open biology 2019 Jun 28;9(6):180263
Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism.
Zulkipli I, Clark J, Hart M, Shrestha RL, Gul P, Dang D, Kasichiwin T, Kujawiak I, Sastry N, Draviam VM
The Journal of cell biology 2018 Sep 3;217(9):3057-3070
The Journal of cell biology 2018 Sep 3;217(9):3057-3070
Pancreatic LKB1 deletion leads to acinar polarity defects and cystic neoplasms.
Hezel AF, Gurumurthy S, Granot Z, Swisa A, Chu GC, Bailey G, Dor Y, Bardeesy N, Depinho RA
Molecular and cellular biology 2008 Apr;28(7):2414-25
Molecular and cellular biology 2008 Apr;28(7):2414-25
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MARK2 Antibody (3B12) [H00002011-M01] - MARK2 monoclonal antibody (M01), clone 3B12. Analysis of MARK2 expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MARK2 Antibody (3B12) [H00002011-M01] - Detection limit for recombinant GST tagged MARK2 is approximately 0.03ng/ml as a capture antibody.